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African Journal of Biotechnology

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Relative expression of genes related with cold tolerance in temperate rice at the seedling stage

GD Nanculao, ML Herrera, MP Carcamo, VB Velasquez

Abstract


Low temperature is one of the main abiotic stresses affecting rice yield in Chile. Alterations in phenology and physiology of the crop are observed after a cold event. The objective of this work was to study the relative expression of genes related with cold stress in Chilean cultivars of rice. For this, we analyzed the expression of candidate genes using real-time polymerase chain reaction (rtPCR), after exposure to cold of the rice cultivars Diamante-INIA and Zafiro-INIA and one experimental line from INIA´s Rice Breeding Program in Chile, Quila 241701, with known high cold tolerance. For analysis, the Spanish cultivar, Susan, was used as check tolerance and Oryzica 1 as susceptible check. Oxidative stress was measured through lipid peroxidation. To find mechanisms of cold tolerance in Chilean cultivars, we determined the relative expression of genes related with oxidative stress, such as superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT). Lipid peroxidation allowed the measurement of the physiological stress level of the genotypes under study. The results indicate that lipid peroxidation in Oryzica 1 was higher than in the other genotypes. No differences were observed in expression levels of gene-encoding SOD and GR between genotypes. Contrary to expected results, high level of the gene-encoding CAT enzyme in Oryzica 1 after cold stress was observed. Future experiment, related with enzymatic activity and non-enzymatic antioxidant mechanism, are necessary to elucidate the relationship between cold stress and expression levels of gene-encoding antioxidant enzyme in Chilean rice germplasm.

Keywords: Cold stress, reactive oxygen species, antioxidant enzymes.

African Journal of Biotechnology Vol 13(25) 2506-2512



http://dx.doi.org/10.5897/AJB2014.13793
AJOL African Journals Online