Composition and antioxidant and antifungal activities of the essential oil from Lippia gracilis Schauer

  • CS Franco
  • AF Ribeiro
  • NCC Carvalho
  • OS Monteiro
  • JKR da Silva
  • EHA Andrade
  • JGS Maia

Abstract

In this study, the oil constituents of Lippia gracilis were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antioxidant and antifungal activities were also evaluated. The leaf oil showed a yield of 3.7% and its main constituents were thymol (70.3%), p-cymene (9.2%), thymol methyl ether (5.4%) and p-methoxythymol (2.7%). The thin stem oil showed a yield of 0.4% and its major components were thymol (70.1%), thymol methyl ether (4.4%), p-methoxythymol (4.0%), p-cymene (3.8%), α-humulene (2.4%) and (E)-caryophyllene (2.1%). The aromatic monoterpenes found in the oils showed an average of 88%. The scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) for the leaf oil, expressed as half maximal effective concentration (EC50), was 35.7±3.3 µg/ml, indicating high antioxidant activity. The evaluation of fungicide activity for the leaf oil, using direct bioautography, showed also a significant value for lethal concentration (LC50 5.0 μg/ml) against Cladosporium sphaerospermum and C. cladosporioides fungi.

Keywords: Essential oil composition, thymol and carvacrol, DPPH radical scavenging and bioautography

African Journal of Biotechnology, Vol 13(30) 3107-3113

Author Biographies

CS Franco
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
AF Ribeiro
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
NCC Carvalho
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
OS Monteiro
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
JKR da Silva
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
EHA Andrade
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
JGS Maia
Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal  do Para, 66075-900, Belem, PA, Brazil
Published
2015-09-01
Section
Articles

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eISSN: 1684-5315