Effect of seasonal collection on callus Induction, proliferation and somatic embryogenesis from anther cultures of Heveabrasiliensis MuellArg

  • W Srichuay
  • S Kalawong
  • S Te-chato

Abstract

Hevea brasiliensis that is grown in Thailand and Southeast Asia is a very valuable source of natural rubber. However, few studies have been conducted to evaluate the effects of seasonal effect on plantlet regenera-tion through somatic embryogenesis from anther culture. Thus, this study discussed the effect of season on callus induction subsequent to plantlet regeneration from culturing of anther. Male flowers were aseptically opened and anthers were excised to culture on callus induction medium (CIM) which was MS supplemented with 5% sucrose, 1 mg.L-1 2,4-D, 1 mg.L-1 KN, 1 mg.L-1 NAA. Somatic embryo (SE) developed on MS medium supplemented with 3% sucrose, 0.2 mg.L-1 NAA, 1 mg.L-1 BA, 3 mg.L-1 KN and 0.05 mg.L-1 GA3. This medium is so called embryo induction medium (EIM).Anther collectedfrom different season gave different result in callus induction, proliferation and somatic embryogenesis, healthy anther collected in February 15, 2013 gave the best result in callus induction (86.25%), proliferation (422 mgFW) and somatic embryogenesis (20%). Complete plantlets (15.63%)were obtained on MS medium supplemented with 5% sucrose, 0.5 mg.L-1 KN, 0.2 mg.L-1 IAA, 1 mg.L-1 BA, 0.3 mg.L-1 GA3 and 0.2% phytagel. Seasonal collection plays influence rolein successful somatic embryogenesis in tissue culture of rubber tree.

Keywords: Hevea brasiliensis, rubber, antherculture, callus induction, seasonal collection, somatic embryogenesis.

African Journal of Biotechnology, Vol 13(31) 3560-3566

Author Biographies

W Srichuay
Faculty of Science and Technology, Princess of Naradhiwas University, Naradhiwat, Thailand; Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University Hatyai, Songkla 90112, Thailand
S Kalawong
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University Hatyai, Songkla 90112, Thailand; Department of Agriculture, Faculty of Science and Technology, BansomdejchaoprayaRajabhat University, Bangkok 10600, Thailand
S Te-chato
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University Hatyai, Songkla 90112, Thailand
Published
2015-09-16
Section
Articles

Journal Identifiers


eISSN: 1684-5315