Antiproliferative and antibacterial activity evaluation of red microalgae Rhodosorus marinus

  • A Garcia-Galaz
  • LE Gutierrez-Millan
  • MA Valdez
  • E Acedo-Felix
  • A Burgos-Hernandez
  • MA Lopez-Torres
  • MGB Zazueta

Abstract

Antiproliferative activity of three extracts obtained from red microalgae Rhodosorus marinus was evaluated against cervical (HeLa), colon (HCT 116), lung (A549), prostate (22Rv-1) and breast (HCC38 and MDA-MB-231) cancer cell lines. Antibacterial activity of these extracts was also tested against Salmonella choleraesuis, Listeria monocytogenes and Staphylococcus aureus. All extracts were obtained from lyophilized biomass of red microalgae. Extract A was obtained using 40% ammonium sulfate precipitation and gel filtration chromatography with G-25 sephadex. Extract B was subjected to a similar process, but 60% ammonium sulfate precipitation was used. Extract C was obtained by methanol extraction and hydrophobicity chromatography using amberlite XAD-2. Protein concentration was determined in two extracts and total phenols in one extract, using Bradford and Folin techniques. Antiproliferative activity was evaluated at extract concentrations ranging from 0.125 to 1 mg/ml, using the spectrophotometric technique MTT (3 - (4,5 - dimetiltiazolyl - 2) - 2,5 - diphenyltetrazolium bromide). The antibacterial activity was evaluated by the impregnated disk test. Extract C showed antiproliferative activity against almost all cancer cell lines with an IC50 of 0.5 (HCT 116), 0.8 (HeLa), 0.9 (MDA-MB-231), 0.1 (HCC38), and 0.4 (22Rv-1) mg/ml, whereas none of the tested extracts showed antibacterial activity under experimental conditions.

Keywords: Rhodosorus marinus, red microalgae, antibacterial activity, antiproliferative activity, methanolic extract. 

African Journal of Biotechnology, Vol 13(43) 4169-4175

Author Biographies

A Garcia-Galaz
Posgrado en Biociencias, Universidad de Sonora, Hermosillo, Mexico
LE Gutierrez-Millan
Departmento de Investigaciones Cientificas y Tecnologicas, Universidad de Sonora, Hermosillo, Mexico
MA Valdez
Departmento de Fisica, Universidad de Sonora, Hermosillo, Mexico
E Acedo-Felix
Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarollo, AC, Hermosillo, Mexico
A Burgos-Hernandez
Departmento de Investigacion y Posgrado en Alimentos, Unversidad de Sonora, Hermosillo, Mexico
MA Lopez-Torres
Departmento de Investigaciones Cientificas y Tecnologicas, Universidad de Sonora, Hermosillo, Mexico
MGB Zazueta
Departmento de Investigaciones Cientificas y Tecnologicas, Universidad de Sonora, Hermosillo, Mexico
Published
2015-10-14
Section
Articles

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eISSN: 1684-5315