An active form of calcium and calmodulin dependant protein kinase (ccamk) of Medicago truncatula

  • S Kassouar
  • MBB Hamed

Abstract

The DMI3 gene of the model legume Medicago truncatula encodes a calcium and calmodulin dependent protein kinase (CCaMK) involved in the signalling pathways leading to the establishment of both mycorrhizal and rhizobial root symbiosis. The removal of the auto-inhibitory domain that negatively regulates the kinase activity in M. truncatula results in a constitutively-active form, inducing symbiotic responses in the absence of bacterial signals. In this study, we verified the functionality of a DMI3 variant and its ability to induce spontaneous nodules in M. truncatula dmi3 mutant. Our results based on enzymatic radio activity assay using [y-33P] ATP, suggests that the DMI3-311 variant is active and its corresponding gene (DMI3-311) when introduced in the dmi3 mutant, by Agrobacterium rhizogenes transformation, induced in the formation of a few spontaneous nodules.

Keywords:  Medicago truncatula, CCaMK; DMI3 variant; spontaneous nodules.

African Journal of Biotechnology, Vol 13(52) 4463-4666

Author Biographies

S Kassouar
Department  de Biotechnologie, Faculte des Sciences de la nature et de la vie, Universite d'Oranx, Oran, Algerie
MBB Hamed
Department  de Biotechnologie, Faculte des Sciences de la nature et de la vie, Universite d'Oranx, Oran, Algerie
Published
2015-11-16
Section
Articles

Journal Identifiers


eISSN: 1684-5315