Responses of selected biota after biostimulation of a vegetable oil spill in the Con Joubert Bird Sanctuary wetland: A pilot study

  • Mapurunyane C Selala
  • Paul J Oberholster
  • Karen AK Surridge
  • Arno R de Klerk
  • Anna-Maria Botha

Abstract

An investigation on the effect of a vegetable oil spill was conducted on the biological diversity of the Con Joubert Bird Sanctuary wetland in South Africa before and after biostimulation with different concentrations of fertilizer during 2008. Biostimulation responses were analyzed 30 days after different concentrations of fertilizer were applied to the freshwater wetland at three selected sampling sites. The Con Joubert Bird Sanctuary wetland showed a high degree of contamination after a vegetable oil spill, resulting in a large volume of vegetable oil in the sediment and water column, respectively. Vegetable oil contents differed at each sampling site before biostimulation and each site showed variable responses after biostimulation. In this study, biostimulation results displayed a high yield of microbial activity and vegetable oil degradation at site one and two respectively. However, the degradation of the high vegetable oil concentrations within the sediments at sampling site 3 may have been hampered or retarded by the polymerized state of the vegetable oil. The phytoplankton, protozoan, macroinvertebrates and microorganisms assemblage were affected and showed little improvement at site 3, even after biostimulation with the high fertilizer concentration of 800 g/m2, in comparison to sites 1 and 2 which showed greater biological activities and degradation of vegetable oil.

Keywords: Biostimulation, vegetable oil spill, fresh water wetland

African Journal of Biotechnology Vol. 12(4), pp. 385-399

Author Biographies

Mapurunyane C Selala
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria,
P/Bag X04, Onderstepoort 0110, South Africa
Paul J Oberholster
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria,
P/Bag X04, Onderstepoort 0110, South Africa; CSIR Natural Resources and the Environment, P.O. Box 320, Stellenbosch 7599, South Africa
Karen AK Surridge
Department of Soil Science, University of Pretoria, Hillcrest Pretoria Private Bag X20 Hatfield PRETORIA 0028 South Africa
Arno R de Klerk
CSIR Natural Resources and the Environment, P.O. Box 320, Stellenbosch 7599, South Africa; Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7601 South Africa
Anna-Maria Botha
CSIR Natural Resources and the Environment, P.O. Box 320, Stellenbosch 7599, South Africa; Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7601 South Africa
Published
2015-11-29
Section
Articles

Journal Identifiers


eISSN: 1684-5315