Callus induction and regeneration of elite Indian maize inbreds

Abstract

Five elite Indian maize inbreds namely; HKI1105, HKI1105, HKI335, CM300 and LM5 were evaluated for callus induction and regeneration. Immature embryos obtained 14 days after pollination were used as explants. Genotype, medium, type of auxin and their concentrations influenced callus induction. N₆ medium supplemented with different concentration of 2,4-D (1, 2 and 3 mg/l) and Dicamba (1, 2 and 3 mg/l) were used for callus induction. N₆ supplemented with 2 mg/l of 2,4-D has shown highest percentage of embryogenic callus induction. Among the five genotypes tested, CM300 gave highest percentage of embryogenic calli. CM300 and LM5 both have shown higher regeneration percentage of 12.22%.

Key words: Maize, in-vitro culture and regeneration.