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African Journal of Biotechnology

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A more improved protocol for in vitro shoot organogenesis in daylily (Hemerocallis sp.)

Kanyand Matand, Ning Wu, Stephan Conley, George Acquaah

Abstract


Daylily is a crop of landscape, nutritional, and medicinal importance. Despite relative progress, daylily is one of the least explored crops in tissue culture, partly, because of its low responsiveness for in vitro organogenesis. This study reports a more efficient one-step protocol for shoot organogenesis. Leaf, flower, flower bud, stem, and root tissues were investigated for shoot organogenesis after different growth regulator treatments on Murashige and Skoog medium. Three out of four of the daylily formed shoots. The variety 'Summer Echoes' was the best of all the other varieties with the greatest average shoots per explant (118) and shooting explant rate 87% when 6-benzyl-aminopurine (BA) and kinetin were used singly. This protocol is the most improved, over the current ones, for shoot organogenesis in daylily. The plant grew normally in the greenhouse.

Keywords: Daylily shoots organogenesis, plant micropropagation, daylily tissue culture, in vitro plant regeneration

African Journal of Biotechnology Vol. 12(8), pp. 820-825



AJOL African Journals Online