Co-extraction of high-quality RNA and DNA from rubber tree (Hevea brasiliensis)

  • Zewei An
  • Qitong Wang
  • Yanshi Hu
  • Yanhong Zhao
  • Yacao Li
  • Han Cheng
  • Huasun Huang
Keywords: Hevea brasiliensis, polyethylene glycol (PEG), nucleic acid, co-extraction, higher plants.

Abstract

High-quality nucleic acids are the basic requirement for performing genomic research. A reliable and efficient method was developed for co-extracting high-quality DNA and RNA from rubber tree (Hevea brasiliensis) in this study. Polyethylene glycol (PEG) and cetyltrimethylammonium bromide (CTAB) extraction buffer with high concentrations of polyvinylpyrrolidone (PVP) and β-mercaptoethanol was used in this study. The results show that 3.2% polyethylene glycol 8000 is the optimal concentration for successful separation of DNA and RNA. Spectrophotometric determination (A260/A280 and A260/A230 ratios), agarose electrophoresis analysis and reverse transcription (RT-PCR) of isolated nucleic acids indicate that high-quality DNA and RNA were extracted by this method. The general applicability of this method was also evaluated, and the results show that it was suitable for a variety of plants.

Key words: Hevea brasiliensis, polyethylene glycol (PEG), nucleic acid, co-extraction, higher plants.

Section
Articles

Journal Identifiers


eISSN: 1684-5315