Analysis of TNF-a and IL-10 gene polymorphisms in Zimbabwean children exposed to malaria

  • Takafira Mduluza
  • Elizabeth Gori
  • Paradzai Chitongo
  • Nicholas Midzi
  • Tinashe Ruwona
  • White Soko
  • Godfree Mlambo
  • Susan L Mutambu
  • Nirbhay Kumar

Abstract

Single nucleotide polymorphisms within the cytokine genes, TNF-α (-308 G/A), and IL-10 (-1082 A /G and -819 T/C) associated with protection and susceptibility to parasitic infections were examined in samples from school aged children in the Eastern district of Zimbabwe. Whole blood specimens were obtained from 491 children between the ages of 5 – 16 years, of which 26.9% were infected with Plasmodium falciparum and 73.1% were not infected. Genotyping was carried out using the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The prevalence of TNF-α genotypes GG, GA and AA associated with low, intermediate and high cytokine production was 80, 19 and 2%, respectively. Wild type alleles TNF-α -308G* predominated in both infected and uninfected individuals in the study. For IL-10 (position -819) the distribution of wild-type alleles CC*, heterozygotes and mutant carriers TT* was 64, 27 and 9%, respectively, and a similar analysis of the polymorphisms on position -1082 for IL-10 revealed that most of the samples were heterozygotes (95%). There was no statistically significant difference in the frequencies of polymorphisms on TNF-α (-308G/A) (X2 = 1.820; p = 0.403), IL-10 (-1082 A/G) (X2 = 0.242; p = 0.623) and IL-10 (-819C/T) among children infected with P. falciparum and those without infection. Finally, the high prevalence of heterozygotes would suggest moderate to high IL-10 responses in the population analyzed and that an anti-inflammatory environment dominates when faced with acute P. falciparum infection in the samples analyzed.

Keywords: Polymorphism, cytokines, Plasmodium falciparum, malaria

African Journal of Biotechnology Vol. 12(10), pp. 1034-1039, 6 March, 2013

Author Biographies

Takafira Mduluza
The Scripps Research Institute, Department of Immunology & Microbial Sciences, La Jolla, CA, USA
Elizabeth Gori
Biochemistry Department,University of Zimbabwe, Harare, Zimbabwe
Paradzai Chitongo
Biochemistry Department,University of Zimbabwe, Harare, Zimbabwe
Nicholas Midzi
Biochemistry Department,University of Zimbabwe, Harare, Zimbabwe; National Institute of Health Research, Ministry of Health & Child Welfare, Harare, Zimbabwe
Tinashe Ruwona
The Scripps Research Institute, Department of Immunology & Microbial Sciences, La Jolla, CA, USA
White Soko
Biochemistry Department,University of Zimbabwe, Harare, Zimbabwe; National Institute of Health Research, Ministry of Health & Child Welfare, Harare, Zimbabwe
Godfree Mlambo
Molecular Microbiology & Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States
Susan L Mutambu
National Institute of Health Research, Ministry of Health & Child Welfare, Harare, Zimbabwe
Nirbhay Kumar
Tulane School of Public Health & Tropical Medicine, Tulane University, New Orleans, USA
Published
2016-01-05
Section
Articles

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eISSN: 1684-5315