Biochemical aspects of single-node cuttings of Ricinodendron heudelotii (Baill.) in relation with rooting

  • Nehemie Donfagsiteli Tchinda
  • Hubert Jean Claude Mbita Messi
  • Fotso Fotso
  • Germo Nzweundji
  • Doungous Oumar
  • Bernard Dongmo
  • Sanonne Sanonne
  • Gabriel Agbor Agbor
  • Denis Omokolo Ndoumou

Abstract

Ricinodendron heudelotii (Njansang) is a valuable multipurpose tree species retained for domestication in Central and Western African regions. To measure the ability of rooting in relation with biochemical changes, basal single-node leafy cuttings were treated with different concentrations of indole-3-butyric acid (IBA), and 1-naphthaleneacetic acid (NAA) and cultured in fine sand media under poly-propagator system. The adventitious rooting was obtained in three distinct stages: Induction (0 to 20 days), initiation (20 to 30 days) and expression (30 to 40 days). Rooting response was higher within nodal cuttings pretreated with IBA than those pretreated with NAA. Polyphenoloxidase activity started to increase both in treated and control cutting during the initiation stage of the experiment and decreased after root emergence only in treated cuttings. Indole-3 acetic acid (IAA)-oxidase activity of auxin treated cuttings decreased as compared to the control. The peroxidase activity in IBA-treated cuttings increased slowly at the initiation stages and lightly at the expression stage. Total phenolic content was higher in IBA-treated cutting particularly at the initiation and expression stages. Phenolics and
polyphenoloxidase might be playing key role for emergence of adventitious rooting and can be used as rooting enhancer in R. heudelotii.

Keywords: Auxins, enzyme activity, nodal cutting, Ricinodendron heudelotii, vegetative propagation

African Journal of Biotechnology Vol. 12(10), pp. 1049-1056

Author Biographies

Nehemie Donfagsiteli Tchinda
Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P.O. Box 6163 Yaounde, Cameroon
Hubert Jean Claude Mbita Messi
Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P.O. Box 6163 Yaounde, Cameroon
Fotso Fotso
Laboratory of General Biology, Higher Teacher Training College. P. O. Box 42 Bambili, Cameroon
Germo Nzweundji
Laboratory of General Biology, Higher Teacher Training College. P. O. Box 42 Bambili, Cameroon
Doungous Oumar
Institute of Agricultural Research for Development, Wakwa, P. O. Box 65 Ngaoundere, Cameroon
Bernard Dongmo
Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P.O. Box 6163 Yaounde, Cameroon
Sanonne Sanonne
Department of Life and Earth Sciences, Higher Teacher Training College, University of Maroua, P. O. Box 55
Cameroon
Gabriel Agbor Agbor
Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P.O. Box 6163 Yaounde, Cameroon
Denis Omokolo Ndoumou
Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P.O. Box 6163 Yaounde, Cameroon
Published
2016-01-13
Section
Articles

Journal Identifiers


eISSN: 1684-5315