In vitro antifungal activity of Argemone ochroleuca Sweet latex against some pathogenic fungi

  • Mahmoud FM Moustafa
  • Saad A Alamri
  • Tarek H Taha
  • Sulaiman A Alrumman

Abstract

The in vitro antifungal activities of crude latex of Argemone ochroleuca Sweet against four clinical isolates of Candida (Candida albicans, Candida glabrata, Candida krusei and Candida tropicalis) and six isolates of plant pathogenic fungi (Alternaria alternate, Drechslera halodes, Fusarium oxysporum, Macrophomina phaseolina, Pythium ultimum and Rhizoctoina solani) were assessed using well diffusion method. The chemical compounds of the hexan extract of A. ochroleuca latex were investigated using Perkin-Elmer Gas chromatography-Mass spectrometry. The latex of A. ochroleuca showed antifungal activity against D. halodes (10.60 mm) and Candida spp. (15.06 to 20.16 mm). GC-MS analysis of the hexan extract of A. ochroleuca latex revealed that the latex contains diethyl phthalate (81.57%), 6-Nitro-imidazo(1,2-a)pyridine compound (8.833%), cyclohexasiloxane, dodecamethyl- (5.607%), 4-(2,2-Dimethyl-6-methylenecyclohexylidene)-3-methylbutan-2-one compound (2.410%) and cycloheptasiloxane, tetradecamethyl- (1.574%). These findings indicate that the latex of A. ochroleuca could be a good source of antifungal agent against D. halodes and Candida spp. and the phytocomponents present in the latex could be used against fungal pathogens.

Keywords: Argemone ochroleuca, latex, pathogenic fungi, GC-MS analysis

African Journal of Biotechnology Vol. 12(10), pp. 1132-1137

Author Biographies

Mahmoud FM Moustafa
Department of Botany, Faculty of Science, South Valley University, Qena, Egypt; Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia
Saad A Alamri
Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia
Tarek H Taha
Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia; Environmental BiotechnologyDep., GEBRI Institute, City of Scientific Research and Technology Applications, B. O., 21934, New Borg El-Arab City, Aexandria, Egypt
Sulaiman A Alrumman
Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia
Published
2016-01-13
Section
Articles

Journal Identifiers


eISSN: 1684-5315