Protective effect and expression of defense-related ESTs induced by acibenzolar-S-methyl and a phosphorylated mannan oligosaccharide-based product against Moniliophthora perniciosa in Theobroma cacao

  • Lívia de Matos Pereira
  • Mário Lúcio Vilela de Resende
  • Sandra Marisa Mathioni
  • Magnólia Araújo Campos
  • Pedro Martins Ribeiro Júnior
  • Fabrício Rabelo Camilo
  • Anderson Tadeu Silva
  • Luciano Vilela Paiva

Abstract

Witches’ broom disease (WBD), caused by the fungus Moniliophthora perniciosa, is one of the main diseases in cocoa (Theobroma cacao) and has caused severe economic losses. Integrated disease management has been the focus for its control and therefore, the identification of new inducers of plant resistance is desirable. Thus, the goal of this work was to evaluate two potential inducers of resistance against WBD. A phosphorylated mannan oligosaccharide-based product (PMO) and acibenzolar S-methyl (ASM) were tested on M. perniciosa inoculated seedlings and in field experiments and showed a reduction on the incidence of WBD. The expression of two defense-related expressed sequence tags (ESTs) in cocoa, coding for peroxidase (Pox) and chitinase (Chi), were accessed by qPCR. Both products induced the expression of the Pox defense-related EST. In general, ASM induced the expression of chitinase (Chi) and peroxidase (Pox) in earlier time-points than PMO. However, PMO provided long-lasting and higher levels of expression. Chi expression was triggered in the time-points succeeding the spraying but was very low. On the other hand, peaks of Pox transcripts were detected in later time-points for both inducers. ASM and PMO modes of action might be explained, at least partially, by the overexpression of defense-related ESTs.

Keywords: Cocoa, witches’ broom disease, disease control, peroxidase, chitinase, induced resistance, elicitors, quantitative reverse transcriptase PCR (qRT-PCR)

African Journal of Biotechnology Vol. 12(12), pp. 1311-1317

Author Biographies

Lívia de Matos Pereira
Departamento de Fitopatologia, Universidade Federal de Lavras, CP 3037, CEP 37.200-000, Lavras, MG, Brazil
Mário Lúcio Vilela de Resende
Departamento de Fitopatologia, Universidade Federal de Lavras, CP 3037, CEP 37.200-000, Lavras, MG, Brazil
Sandra Marisa Mathioni
Departamento de Fitopatologia, Universidade Federal de Lavras, CP 3037, CEP 37.200-000, Lavras, MG, Brazil
Magnólia Araújo Campos
Unidade Acadêmica de Educação-Biologia, Universidade Federal de Campina Grande, Centro de Educação e Saúde, CEP 58175-000, Cuité, PB, Brazil
Pedro Martins Ribeiro Júnior
Departamento de Fitopatologia, Universidade Federal de Lavras, CP 3037, CEP 37.200-000, Lavras, MG, Brazil
Fabrício Rabelo Camilo
Departamento de Fitopatologia, Universidade Federal de Lavras, CP 3037, CEP 37.200-000, Lavras, MG, Brazil
Anderson Tadeu Silva
Laboratório Central de Biologia Molecular, Universidade Federal de Lavras, MG, Brazil
Luciano Vilela Paiva
Laboratório Central de Biologia Molecular, Universidade Federal de Lavras, MG, Brazil
Published
2016-01-17
Section
Articles

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eISSN: 1684-5315