High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

  • Samia Berraies
  • Mohamed Salah Gharbi
  • François Belzile
  • Amor Yahyaoui
  • Mohamed Rebah Hajlaoui
  • Mokhtar Trifi
  • Martine Jean
  • Salah Rezgui

Abstract

Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of M. graminicola in Tunisia.

Keywords: Durum wheat, genetic diversity, microsatellites, Mycosphaerella graminicola, micro geographical scale, Tunisia

African Journal of Biotechnology Vol. 12(12), pp. 1344-1349

Author Biographies

Samia Berraies
Laboratoire de génétique moléculaire, immunologie et biotechnologie Faculté des Sciences de Tunis, Tunisie
Mohamed Salah Gharbi
Département de Phytologie, Pavillon Charles-Eugene-Marchand, Faculté des sciences de l’agriculture et de l’alimentation, Université Laval, Québec, Canada
François Belzile
Département de Phytologie, Pavillon Charles-Eugene-Marchand, Faculté des sciences de l’agriculture et de l’alimentation, Université Laval, Québec, Canada
Amor Yahyaoui
International Maize and Wheat Improvement Center (CIMMYT) Mexico, Mexico
Mohamed Rebah Hajlaoui
Laboratoire de Biotechnologie Agricole et Alimentaire. Institut National de Recherche Agronomique de Tunis, Rue Hédi Karray, 2049, Tunis Tunisie
Mokhtar Trifi
Laboratoire de génétique moléculaire, immunologie et biotechnologie Faculté des Sciences de Tunis, Tunisie
Martine Jean
Département de Phytologie, Pavillon Charles-Eugene-Marchand, Faculté des sciences de l’agriculture et de l’alimentation, Université Laval, Québec, Canada
Salah Rezgui
Laboratoire de Génétique et d’Amélioration des Plantes, Institut National Agronomique de Tunis, Tunisie.
Published
2016-01-17
Section
Articles

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eISSN: 1684-5315