Molecular marker analysis to differentiate a clonal selection of Centennial Seedless grapevine

  • Manisha P Shinde
  • Anuradha Upadhyay
  • Lalitkumar B Aher
  • GS Karibasappa

Abstract

Microsatellite and amplified fragment length polymorphism (AFLP) markers were used to differentiate Manjari Naveen, a clonal selection of Centennial Seedless variety of grape. Twenty one (21) microsatellite primers could not detect variation between parent variety and its clone. AFLP analysis with 40 primer combinations generated 1093 markers. Three AFLP markers were polymorphic; two markers were present only in Centennial Seedless, whereas one was present only in Manjari Naveen. These markers will be useful for establishing genetic identity, variety registration and protection of breeder’s right.

Keywords: Vitis vinifera, AFLP, microsatellites, clonal selection

African Journal of Biotechnology Vol. 12(14), pp. 1594-1597

Author Biographies

Manisha P Shinde
National Research Centre for Grapes, Solapur Road, Pune -412307, Maharashtra, India.
Anuradha Upadhyay
National Research Centre for Grapes, Solapur Road, Pune -412307, Maharashtra, India.
Lalitkumar B Aher
National Research Centre for Grapes, Solapur Road, Pune -412307, Maharashtra, India.
GS Karibasappa
National Research Centre for Grapes, Solapur Road, Pune -412307, Maharashtra, India.
Published
2016-01-20
Section
Articles

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eISSN: 1684-5315