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African Journal of Biotechnology

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DNA fingerprinting of spore-forming bacterial isolates, using Bacillus cereus repetitive polymerase chain reaction analysis (Bc-Rep-PCR)

N Du Rand, MD Laing

Abstract


Bc-repetitive extragenic palindromic polymerase chain reaction (Bc-Rep PCR) analysis was conducted on seven Bacillus thuringiensis isolates accessed from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) culture collection and on five local isolates of entomopathogenic spore-forming bacteria. The five isolates were three strains of B. thuringiensis, one strain of B. cereus and one strain of Brevibacillus laterosporus. All five isolates were distinguished from each other using Bc-Rep PCR analysis. The three B. thuringiensis isolates were closely related. The isolate of B. laterosporus was not related to any of the B. cereus group isolates. Serotyping was also conducted on the five local isolates. However, only one of these isolates could be identified with serotyping and was identified as B. thuringiensis subsp. kenyae.

Keywords: Bc-repetitive extragenic palindromic polymerase chain reaction, Bacillus sp., serotyping

African Journal of Biotechnology Vol. 12(14), pp. 1598-1603



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