Evaluation of growth performance and nutritional quality of diets using digestive enzyme markers and in vitro digestibility in Siamese fighting fish (Betta splendens Regan, 1910)

  • Karun Thongprajukaew
  • Uthaiwan Kovitvadhi
  • Satit Kovitvadhi
  • Arunee Engkagul
  • Krisna Rungruangsak-Torrissen

Abstract

Digestive enzymes and their effects on in vitro digestibility of feeds and feedstuffs, as well as on growth performance quality were studied in Siamese fighting fish (Betta splendens Regan, 1910). The specific activities of total protease, amylase, trypsin and chymotrypsin increased during development and were higher in females than in males at maturation (P < 0.05). The activity ratio of trypsin to chymotrypsin corresponded to fish growth, and showed lower values in females than in males. White muscle levels of RNA decreased during development, with females having higher levels than males, while the protein levels increased with no difference between sexes. In the oocytes, trypsin-like and chymotrypsin-like specific activities were very low, and the concentrations of RNA, protein and protein/lipid ratio were higher than in the muscle. For in vitro digestibility, wheat gluten, soybean meal and fish meal were among good protein sources while the meals from peanut, fish and soybean were good carbohydrate sources. Golden apple snail meat was a good source for both protein and carbohydrate. The crude enzyme extracts from different growth stages and sexes had different abilities to digest the same feeds and feedstuffs. This will make it possible to preliminarily study the authenticated nutritional quality of raw materials for future feed formulations for B. splendens.

Keywords: Betta splendens, digestive enzymes, feedstuff, in vitro digestibility, muscle quality, oocyte quality, Siamese fighting fish

African Journal of Biotechnology Vol. 12(14), pp. 1689-1702

Author Biographies

Karun Thongprajukaew
Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Biochemical Research Unit for Feed Utilization Assessment, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of Applied Science, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand
Uthaiwan Kovitvadhi
Biochemical Research Unit for Feed Utilization Assessment, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Satit Kovitvadhi
Department of Agriculture, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok 10600, Thailand
Arunee Engkagul
Biochemical Research Unit for Feed Utilization Assessment, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Krisna Rungruangsak-Torrissen
Biochemical Research Unit for Feed Utilization Assessment, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Institute of Marine Research, Ecosystem Processes Research Group, Matre Research Station, N-5984 Matredal, Norway
Published
2016-01-20
Section
Articles

Journal Identifiers


eISSN: 1684-5315