Purification and characterization of angiotensin-1 converting enzyme (ACE)-inhibitory peptide from the jellyfish, Nemopilema nomurai

  • Chi-Won Lim
  • Yeon-Kye Kim
  • So-mi Yeun
  • Moon-Hee Lee
  • Ho-Sung Moon
  • Na-Young Yoon
  • Ho-Dong Yoon
  • Hee-Yeon Park
  • Doo-Seog Lee

Abstract

The Nemopilema nomurai hydrolysate was produced by the reaction of papain, and an angiotensin-Ι converting enzyme (ACE)-inhibitory peptide was purified by using the molecular cut-offs membrane filter, the gel filtration chromatography with Sephadex LH-20 and the reverse phase chromatographic method using C18 and C12 columns. Purification yield of the active peptide was estimated to be 0.2 ± 0.1%, starting from the lyophilized jellyfish. The infrared (IR), proton nuclear magnetic resonance spectroscopy (1H NMR), carbon nuclear magnetic resonance (13C NMR) and mass spectrometry (MS) spectrometer analyses elucidated that the structure of the purified peptide is tyrosine-isoleucine (Tyr-Ile). The inhibitory concentration at 50% (IC50) and Ki values were calculated to be 2.0 ± 0.3 μg/ml and 3.3 ± 0.3 μM, respectively, which acts as a competitive inhibitor to ACE.

Keywords: Angiotensin-Ι converting enzyme, Jellyfish, Nemopilema nomurai, Papain hydrolysate, Tyrosine-Isoleucine

African Journal of Biotechnology Vol. 12(15), pp. 1888-1893

Author Biographies

Chi-Won Lim
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Yeon-Kye Kim
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea; Aquaculture Industry Division, SSFRI, National Fisheries Research and Development Institute (NFRDI), Yeosu, Jeonnam 556-823, Korea
So-mi Yeun
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Moon-Hee Lee
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Ho-Sung Moon
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Na-Young Yoon
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Ho-Dong Yoon
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Hee-Yeon Park
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Doo-Seog Lee
Food and Safety Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea
Published
2016-01-20
Section
Articles

Journal Identifiers


eISSN: 1684-5315