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African Journal of Biotechnology

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Purification, composition analysis and antioxidant activity of different polysaccharides from the fruiting bodies of Pholiota adiposa

Yongxin Nie, Hongxia Jiang, Yanyou Su, Changxiang Zhu, Jiarui Li, Fujiang Wen

Abstract


Three water-soluble polysaccharide fractions (PAP1-1, PAP1-2 and PAP2-1) were isolated and purified from the fruiting bodies of Pholiota adiposa by diethylaminoethanol (DEAE) Sepharose fast flow column and superdexTM 200 column chromatography on an AKTA Purifier system. Results of high-performance size-exclusion  chromatography (HPSEC) indicated that the average molecular weights of PAP1-1, PAP1-2 and PAP2-1 were 2.3 x 106 Da, 8.8 x 103 Da and 2.1 x 106 Da, respectively. Monosaccharide compositions analysis revealed that PAP1-1 and PAP2-1 were both mainly composed of glucose, and PAP1-2 was composed of rhamnose and  glucose with a mole ratio of 1:3.61. The evaluation of antioxidant activity suggested that PAP1-1 with  β-configuration glycosidic bond exhibited stronger scavenging activity of superoxide radical and hydroxyl  radical than PAP1-2 and PAP2-1 contained both α- and β-configuration glycosidic bond, and should be  explored as a novel potential antioxidant. Available data obtained with in vitro models suggested that  antioxidant activity of polysaccharide may be related to the configuration of glycosidic bond.


Key words: Pholiota adipose, polysaccharide, purification, antioxidant activity.




http://dx.doi.org/10.5897/AJB11.3117
AJOL African Journals Online