Micropropagation of <i>Anthurium andreanum</i> cv. Terra

Abstract

Anthurium anderanum cv. Terra from family Araceae, is a plant with a high commercial value. Conventional propagation of this plant performed via suckers and seeds planting that nowadays missed its application have been abolished. This study aimed to establish producible protocol for indirect in vitro regeneration of the Terra genotype. For this purpose, the effects of 16 different plant growth regulators treatments on callus induction and regeneration of leaf explants were studied. Callus with the highest fresh and dry weight was produced on modified Murashige and Skoog medium containing 0.1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 1.5 mg/L 6-benzylaminopurine (BAP). For regeneration stage, induced calli were transferred to modified MS medium without plant growth regulators, and after six weeks, shoots formed. For rooting, regenerated shoot were transferred to MS medium without any plant growth regulators. Regenerated plantlets potted on mixture of coco pit and perlite were succesfully acclimated.

Key words: Anthurium, micropropagation, growth regulators, regeneration.