In vitro micro-propagation of Longiflorum-Asiatic (LA) hybrids lily (<i>Lilium</i>) cultivar ‘eyeliner’

Abstract

Bulblets propagation by tissue culture was one of the key techniques in the production of lily (Lilium) bulbs. Therefore, in vitro micro propagation of lily bulblets was studied in detail in this paper. L A hybrids lily cultivar ‘eyeliner’ was selected as the materials. By using the method of orthogonal design, the following were concluded from the research: the optimum treatment and disinfection methods of ‘eyeliner’ bulb scales was soaking in 1:500 carbendazim solution for 30 min, disinfection in 75% alcohol for 10 to 60 s, disinfection in 2% NaClO solution for 15 min; the optimum medium for bud induction of ‘eyeliner’ was MS + 0.5 mg·L^-1 6-benzyl aminopurine (6-BA) + 0.1 mg·L^-1 naphlene acetic acid (NAA) + 90 g·L^-1 sucrose, and 25°C and in darkness; the optimum medium for bulblets induction of ‘eyeliner’ was 2MS + 1.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + sucrose 90 g·L^-1 + Paclobutrazol (PP333) 2 mg·L^-1; the optimum culture condition for bulblets induction of ‘eyeliner’ was 20°C, 14 h·day^-1 lightness + 10 h·day^-1 darkness. The optimum medium for rooting culture of ‘eyeliner’ was ½ MS + 0.8 mg·L^-1 NAA + 3 g·L^-1 activated charcoal, 20°C, 14 h·day^-1 lightness + 10 h·day^-1 darkness.

Keywords: Lily bulb, orthogonal experiment, in vitro micro propagation