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African Journal of Biotechnology

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Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs

Nguyen Trong Ngu, Nguyen Thiet, Le Trung Kien, Chau Thanh Vu, Nguyen Thi Hong Nhan, Pham Ngoc Du, Nguyen Thi Kim Khang, Tran Nhan Dung

Abstract


Calpastain (CAST) activity plays a major role in muscle growth and proteolytic changes post-mortem and the CAST gene has been considered as a candidate gene for carcass and pork quality characteristics. The aim of this study was to analyze the association of two polymorphisms namely CAST_HinfI (allele A and B) and CAST_MspI (allele C and D) with carcass and meat quality traits in Mongcai, a Vietnamese indigenous pig breed. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to genotype the animals at these loci. Results indicate that the CAST_HinfI single nucleotide polymorphism (SNP) had a low frequency of allele A as compared to allele B, while the C and D allele distribution was almost the same for the CAST_MspI SNP. In the association analysis, significant effects on dressing percentage of carcass were detected. The CAST_HinfI locus was associated with the pH24, while the CAST_MspI position was in association with pH45 min, drip loss48 and redness color. Additional analysis showed a variation in muscle fiber type composition with higher proportion of IIx fiber in pigs with AB genotype (P < 0.05). Three constructed haplotypes namely AB/CD, AB/DD and BB/CC also had significant effects on carcass, type IIa and IIb fiber percentages.

Keywords: Association, carcass, pork quality, Vietnamese local pig




http://dx.doi.org/10.5897/AJB12.2022
AJOL African Journals Online