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African Journal of Biotechnology

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Characterisation of human coronavirus-NL63 nucleocapsid protein

Michael Berry, Taryn-Lee Manasse, Yee-Joo Tan, Burtram C. Fielding

Abstract


Coronavirus N is a multifunctional protein that plays an essential role in enhancing the efficiency of virus transcription and assembly. This manuscript reports the analysis of HCoV-NL63 N protein by comparing the amino acid sequences of coronavirus N-homologues. A ~50 kDa protein was expressed in both a mammalian cell and bacterial cell system that is similar in size to the predicted ~42.6 kDa HCoV-NL63 N protein. PSORTII identified two putative nuclear localisations signals and PONDR identified one disordered region in HCoV-NL63 N. The reported protein analysis serves as a prelude to laboratory analysis to understand the processing of HCoV-NL63 N.

Keywords: HCoV-NL63, nucleocapsid protein, protein analysis, nuclear localisation signals, protein ordered/disordered regions




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