Characterisation of human coronavirus-NL63 nucleocapsid protein

  • Michael Berry
  • Taryn-Lee Manasse
  • Yee-Joo Tan
  • Burtram C. Fielding
Keywords: HCoV-NL63, nucleocapsid protein, protein analysis, nuclear localisation signals, protein ordered/disordered regions

Abstract

Coronavirus N is a multifunctional protein that plays an essential role in enhancing the efficiency of virus transcription and assembly. This manuscript reports the analysis of HCoV-NL63 N protein by comparing the amino acid sequences of coronavirus N-homologues. A ~50 kDa protein was expressed in both a mammalian cell and bacterial cell system that is similar in size to the predicted ~42.6 kDa HCoV-NL63 N protein. PSORTII identified two putative nuclear localisations signals and PONDR identified one disordered region in HCoV-NL63 N. The reported protein analysis serves as a prelude to laboratory analysis to understand the processing of HCoV-NL63 N.

Keywords: HCoV-NL63, nucleocapsid protein, protein analysis, nuclear localisation signals, protein ordered/disordered regions

Published
2016-01-26
Section
Articles

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eISSN: 1684-5315