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Antioxidant activity and acute toxicity of <i>Neoglaziovia variegata</i> (Bromeliaceae)


Sarah Raquel Gomes de Lima-Saraiva
Amanda Leite Guimarães
Ana Paula de Oliveira
Henrique César Costa Saraiva
Raimundo Gonçalves de Oliveira-Júnior
Vanessa Raquel P. de Barros
Vanúzia G. Menezes
Roniere Alencar de Oliveira
Fabrício Sousa Silva
Ricardo Santana de Lima
Maria Helena Tavares de Matos
Elba Lúcia Cavalcanti Amorim
Jackson Roberto Guedes da Silva Almeida

Abstract

Antioxidant activities of Neoglaziovia variegata were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching and was compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The total phenolics content of the extracts was determined by the Folin-Ciocalteu method. Total flavonoid was also determined. The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50 5.08 ± 0.20 μg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice. No mortality and no toxicity signs were observed, indicating low toxicity of the extract. Blood was removed after 14 days for laboratory analysis of hematological and biochemical parameters. Alterations of aspartate aminotransferase (AST) and creatinine were observed. The data obtained showed that the doses induced microscopic alterations in the liver and kidney. In conclusion, the Nv-EtOH can be considered of low toxicity.

Keywords: Antioxidant activity, acute toxicity, Neoglaziovia variegata, Bromeliaceae


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eISSN: 1684-5315