PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register



Antioxidant activity and acute toxicity of Neoglaziovia variegata (Bromeliaceae)

Sarah Raquel Gomes de Lima-Saraiva, Amanda Leite Guimarães, Ana Paula de Oliveira, Henrique César Costa Saraiva, Raimundo Gonçalves de Oliveira-Júnior, Vanessa Raquel P. de Barros, Vanúzia G. Menezes, Roniere Alencar de Oliveira, Fabrício Sousa Silva, Ricardo Santana de Lima, Maria Helena Tavares de Matos, Elba Lúcia Cavalcanti Amorim, Jackson Roberto Guedes da Silva Almeida

Abstract


Antioxidant activities of Neoglaziovia variegata were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching and was compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The total phenolics content of the extracts was determined by the Folin-Ciocalteu method. Total flavonoid was also determined. The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50 5.08 ± 0.20 μg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice. No mortality and no toxicity signs were observed, indicating low toxicity of the extract. Blood was removed after 14 days for laboratory analysis of hematological and biochemical parameters. Alterations of aspartate aminotransferase (AST) and creatinine were observed. The data obtained showed that the doses induced microscopic alterations in the liver and kidney. In conclusion, the Nv-EtOH can be considered of low toxicity.

Keywords: Antioxidant activity, acute toxicity, Neoglaziovia variegata, Bromeliaceae




http://dx.doi.org/10.5897/AJB12.1913
AJOL African Journals Online