Effect of thermal stress of short duration on the red blood cell parameters of Barbus balcanicus Kotlik, Tsigenopulos, Rab, Berrebi, 2002

  • Dekić Radoslav
  • Ivanc Aleksandar
  • Gnjato Rajko
  • Trbić Goran
  • Ćetković Danijela
  • Lolić Svjetlana

Abstract

In this study, red blood cell parameters of Barbus balcanicus Kotlik, Tsigenopulos, Rab, Berrebi, 2002 subjected to acute thermal stress were investigated. Fish were caught by electro fishing in the Suturlija river, a small tributary of the river Vrbas (N latitude 44˚44' 38˝, E longitude 17˚ 09' 10˝) in summer (July) and transported to the laboratory. Fish were randomly distributed in four aquaria of 30 L each. In all the four aquaria, the water temperature was continuously kept at 19°C by appropriate devices. After period of adaptation (three weeks), half of the fish (24) were used as a control group while the other 24 were subjected to thermal stress by raising the water temperature to 29°C (10°C increase) in 60 min. In both control and thermally treated, fish blood was collected by heart puncture according to “Animal welfare act“. Analyses were performed with native blood, without addition of any anticoagulant and the values of red blood cell count (RBC), haemoglobin concentration, packed cell volume (PCV), Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Haemoglobin Concentration (MCHC) were determined. In the thermally treated fish, the values of PCV and MCV increased (p = 0.0003 and p = 0.0147 respectively), and those of MCHC decreased substantially (p = 0.0001).

Keywords: Haematology, temperature, Barbus balcanicus

African Journal of Biotechnology Vol. 12(18), pp. 2484-2491

Author Biographies

Dekić Radoslav
Faculty of Natural Sciences and Mathematics, University of Banja Luka, Bosnia and Herzegovina
Ivanc Aleksandar
State University of Novi Pazar, Serbia Vuka Karadžića bb, 36 300 Novi Pazar, Serbia.
Gnjato Rajko
Faculty of Natural Sciences and Mathematics, University of Banja Luka, Bosnia and Herzegovina
Trbić Goran
Faculty of Natural Sciences and Mathematics, University of Banja Luka, Bosnia and Herzegovina
Ćetković Danijela
Faculty of Natural Sciences and Mathematics, University of Banja Luka, Bosnia and Herzegovina
Lolić Svjetlana
Faculty of Natural Sciences and Mathematics, University of Banja Luka, Bosnia and Herzegovina
Published
2016-02-01
Section
Articles

Journal Identifiers


eISSN: 1684-5315