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African Journal of Biotechnology

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Comparison of Luffa cylindrica L. sponge discs and Ca-alginate gel beads as immobilized matrices of Saccharomyces cerevisiae for bio-ethanol production from sugarcane molasses

Shuvashish Behera, Ramesh C Ray

Abstract


The production of bio-ethanol from cane molasses (diluted to 15% sugar, w/v) was studied, using the yeast, Saccharomyces cerevisiae immobilized in luffa (Luffa cylindrica L.) sponge discs and in Ca-alginate gel beads. At the end of 96 h fermentation, the ethanol yields were 64.67 ± 0.016 and 65.21 ± 0.030 g/l molasses, with luffa and Ca-alginate entrapped S. cerevisiae cells exhibiting 89.90 ± 0.008 and 91.86 ± 0.072% sugar conversion, respectively. There was no statistically significant difference [Fisher’s least significance difference (LSD)] between the two immobilized systems in both sugar utilization (t = 0.254, p < 0.801) and in ethanol production (t = -0.663, p < 0.513).


Key words: Bio-ethanol, cell immobilization, fermentation, molasses, Saccharomyces cerevisiae.




http://dx.doi.org/10.5897/AJB12.830
AJOL African Journals Online