Induced breeding of Labeo rohita through single application of ovaprim-C at Faisalabad Hatchery, Pakistan

  • Muhammad Naeem
  • Amina Zuberi
  • Muhammad Ashraf
  • Waqas Ahmad
  • Abir Ishtiaq
  • Najam-ul-Hasan Najam-ul-Hasan

Abstract

A study was conducted to observe the effect of intramuscular injection of ovaprim-C on the number of eggs/kg, fertilization rate and hatching percentage at a private fish hatchery and research center at Faisalabad, Pakistan, during May to June 2008, on Labeo rohita (Rohu). Studied fish specimens were spawned successfully following a single dose of injection of ovaprim-C (LH-RH analogue) with 0.4 ml kg-1 for female and 0.05 ml kg-1 for male brooders. Ova and milt were stripped simultaneously and mixture was stirred for 15 to 30 s during which fertilization occurred. Hatching occurred within 18 to 30 h after fertilization. The experiment was conducted in circular spawning tank with 2 m diameter. If it is impossible to determine the absolute and relative fecundity, then these parameters can be determined from the body weight. Average number of eggs/kg, fertilized eggs/kg and hatchlings/kg was 63574, 49067 and 39952, respectively. Overall fertilization and hatchling %age was 77.50% and 81.39% respectively. Wet body weight was observed to have a positive influence on absolute (r=0.983) and relative fecundity (r = 0.910) in log-log scale.

Keywords: Induced spawning, ovaprim-C, fecundity, Labeo rohita.

African Journal of Biotechnology Vol. 12(19), pp. 2722-2726

Author Biographies

Muhammad Naeem
Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800, Pakistan
Amina Zuberi
Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Muhammad Ashraf
Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Waqas Ahmad
Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800, Pakistan
Abir Ishtiaq
Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800, Pakistan
Najam-ul-Hasan Najam-ul-Hasan
Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800, Pakistan
Published
2016-02-07
Section
Articles

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eISSN: 1684-5315