PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register



Characterization and cloning of TMV resistance gene N homologues from Nicotiana tabacum

Jun-Shan Gao, Yan Meng, Nobumitsu Sasaki, Hiromi Kanegae, Naomi Hayashi, Hiroshi Nyunoya

Abstract


Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

Key words: N gene, tobacco mosaic virus (TMV), N homologue, Nicotiana tabacum, hypersensitive response.




http://dx.doi.org/10.5897/AJB10.732
AJOL African Journals Online