Characterization of coriander (<i>Coriandrum sativum</i> L.) varieties using SDS-PAGE and RAPD markers

Abstract

In an experiment, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) were evaluated separately as a tool for characterizing coriander varieties. The SDS-PAGE electrophoresis of total soluble seed proteins revealed a total of 7 bands with Rm value ranging from 0.347 to 0.926. A polymorphism of 71.4% was recorded among 20 varieties of coriander. The maximum dissimilarity value was shown by Sindhu. Varieties like Sadhana, Sindhu, RCr-436 and RCr-684 were observed different from the rest of the coriander varieties whereas, RCr-436 and RCr-684 could not be differentiated from each other. Twenty random primers were used for polymorphism studies based on DNA fingerprinting analysis of eighteen coriander varieties. These primers gave amplification and a total of 9 bands were produced with an average of 4.5 bands per primer. A total of 73 polymorphic bands and 17 monomorphic bands were observed thus, showing 81.1% polymorphism among all the varieties. The highest number of bands was generated by the primer OPC-3 and OPC-6 followed by OPC-2, OPC-7 and OPC-20 while the lowest number of band was produced by OPC-19. The variety GC-2 is highly diverse from other varieties. The dendrogram based on protein electrophoresis grouped the varieties into two major clusters whereas RAPD analysis showed clear-cut difference among coriander varieties. Both techniques grouped coriander varieties like Swathi, CS-6, H.Surbhi and RCr-446 in one cluster.

Key words: Coriandrum sativum L., SDS-polyacrylamide gel electrophoresis, RAPD, varietal characterization.