Streptomyces noboritoensis isolated from rhizosphere soil and its use in controlling banana-tissue culture contaminants

  • SH Mohamed
  • SS El-Helafiy
  • A Ismail Mona
  • AS Sadik

Abstract

In this study, the role of Streptomyces noboritoensis (14) isolated from the rhizosphere of banana plant and having antagonistic activity against the bacterial- and fungal-tissue culture contaminants was determined in vitro. Results show that the filtrate was more effective against the fungal-tissue culture contaminants than the bacterial-tissue culture contaminants. This was indicated when jars were fungifree and bacteria-free after one month and 21 days from incubation, respectively. Results of in vitro application show that the filtrate of S. noboritoensis (14) as a bio-control agent could be used for controlling the contaminants in banana. Data showed that treatment F (sterilized shoots treated with Streptomyces filtrate and cultivated on Streptomyces-inoculated medium) was the most effective followed by treatment E (sterilized shoots untreated with Streptomyces filtrate and cultivated on Streptomyces-inoculated medium). Therefore, the study suggests conducting further studies towards the use of streptomycetes in the biological control in a large scale production.

Keywords: Streptomyces, tissue culture contaminants, antagonistic activities, bio-control

African Journal of Biotechnology Vol. 12(20), pp. 2908-2913

Author Biographies

SH Mohamed
Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, KSA
SS El-Helafiy
Department of Biotechnology, Faculty of Science, Taif University, P.O. Box 888, Taif, KSA
A Ismail Mona
Department of Biotechnology, Faculty of Science, Taif University, P.O. Box 888, Taif, KSA
AS Sadik
Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, KSA; Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, P.O. Box 68 Hadayek Shubra, 11241, Cairo, Egypt
Published
2016-03-01
Section
Articles

Journal Identifiers


eISSN: 1684-5315