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African Journal of Biotechnology

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Streptomyces noboritoensis isolated from rhizosphere soil and its use in controlling banana-tissue culture contaminants

SH Mohamed, SS El-Helafiy, A Ismail Mona, AS Sadik

Abstract


In this study, the role of Streptomyces noboritoensis (14) isolated from the rhizosphere of banana plant and having antagonistic activity against the bacterial- and fungal-tissue culture contaminants was determined in vitro. Results show that the filtrate was more effective against the fungal-tissue culture contaminants than the bacterial-tissue culture contaminants. This was indicated when jars were fungifree and bacteria-free after one month and 21 days from incubation, respectively. Results of in vitro application show that the filtrate of S. noboritoensis (14) as a bio-control agent could be used for controlling the contaminants in banana. Data showed that treatment F (sterilized shoots treated with Streptomyces filtrate and cultivated on Streptomyces-inoculated medium) was the most effective followed by treatment E (sterilized shoots untreated with Streptomyces filtrate and cultivated on Streptomyces-inoculated medium). Therefore, the study suggests conducting further studies towards the use of streptomycetes in the biological control in a large scale production.

Keywords: Streptomyces, tissue culture contaminants, antagonistic activities, bio-control

African Journal of Biotechnology Vol. 12(20), pp. 2908-2913



AJOL African Journals Online