African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register

Influence of mixed culture system on the growth performance of Aspergillus oryzae and Saccharomyces cerevisiae

XY Ge, H Qian, WG Zhang


This study describes a novel strategy to improve the α-galactosidase and invertase production of Saccharomyces cerevisiae by co-cultivating it with Aspergillus oryzae. In the mixed culture, the growth of the both strains was repressed, and the protein synthesis for the yeast cell wall was promoted significantly. As a result, α-galactosidase and invertase activities of the mixed culture reached 85.16 and 561.60 Uml-1, over 9 and 15 fold greater than the values obtained in the cultures of single strain, respectively. During the simultaneous saccharification and fermentation procedure, ethanol with the concentration of 15.2% (v/v) was obtained from soybean sirup in 60 h at 30°C, and the conversion efficiency of total sugar to ethanol was 96.3% of the theoretical ethanol yield.

Keywords: Co-cultivation, Aspergillus oryzae, Saccharomyces cerevisiae, α-galactosidase, invertase

African Journal of Biotechnology Vol. 12(21), pp. 3272-3277

AJOL African Journals Online