Influence of mixed culture system on the growth performance of Aspergillus oryzae and Saccharomyces cerevisiae

  • XY Ge
  • H Qian
  • WG Zhang

Abstract

This study describes a novel strategy to improve the α-galactosidase and invertase production of Saccharomyces cerevisiae by co-cultivating it with Aspergillus oryzae. In the mixed culture, the growth of the both strains was repressed, and the protein synthesis for the yeast cell wall was promoted significantly. As a result, α-galactosidase and invertase activities of the mixed culture reached 85.16 and 561.60 Uml-1, over 9 and 15 fold greater than the values obtained in the cultures of single strain, respectively. During the simultaneous saccharification and fermentation procedure, ethanol with the concentration of 15.2% (v/v) was obtained from soybean sirup in 60 h at 30°C, and the conversion efficiency of total sugar to ethanol was 96.3% of the theoretical ethanol yield.

Keywords: Co-cultivation, Aspergillus oryzae, Saccharomyces cerevisiae, α-galactosidase, invertase

African Journal of Biotechnology Vol. 12(21), pp. 3272-3277

Author Biographies

XY Ge
The State Key Laboratory of Food Science and Technology, School of Food Science, JiangNan University, 1800# Lihu Road, Wuxi - 214122, JiangSu Province, P. R. China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, P. R. China
H Qian
The State Key Laboratory of Food Science and Technology, School of Food Science, JiangNan University, 1800# Lihu Road, Wuxi - 214122, JiangSu Province, P. R. China
WG Zhang
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, P. R. China
Published
2016-03-14
Section
Articles

Journal Identifiers


eISSN: 1684-5315