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African Journal of Biotechnology

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Isolation and characterization of Micromonospora bacteria from various soil samples obtained around Lake Van

Metin Ertaş, Kerem Özdemir, Ekrem Atalan

Abstract


The objective of the study was to isolate and characterize the Micromonospora type bacteria from 24 soil samples collected from different localities around the Lake Van basin and Lake Erçek surroundings. Culturing was carried out on selective media following the completion of various physical-chemical analyses of the soil samples. Purified 65 Micromonospora isolate color grouping and numerical taxonomic analyses were carried out. In addition, complete cell protein profiles of 10 Micromonospora test isolates selected as representative of the color groups for characterization studies were determined via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the obtained results were presented as dendograms. Whereas the pHs of the soil samples were generally at neutral values, their organic material content varied from 4.181 to 15.248%. Colony counts in the isolation study varied between 0.9×104 and 14.3×104 cfu/g soil.

Keywords: Micromonospora, numeric analysis, simple matching coefficient, SDS PAGE

African Journal of Biotechnology Vol. 12(21), pp. 3283-3287



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