Histamine and tyramine production by bacteria isolated from spoiled sardine (Sardina pilchardus)

  • Abderrahmane Houicher
  • Esmeray Kuley
  • Badis Bendeddouche
  • Fatih Özogul

Abstract

Bacterial strains (32) from spoiled sardine were isolated and investigated for their ability to produce histamine and tyramine in histidine and tyrosine decarboxylase broth, respectively by a rapid highperformance liquid chromatography (HPLC) method. The predominant microflora of sardine consisted of the genera Enterobacteriaceae, Pseudomonas, Chryseobacterium, Vibrio, Photobacterium, and Stenotrophomonas. In histidine-enriched broth, the highest levels of histamine were observed in Proteus mirabilis (5201.95 mg/L), followed by Enterobacter cloacae (2333.99 mg/L), whilst the lowest histamine accumulation was found for Kluyvera spp. and Listeria monocytogenes at the level of 0.38 and 0.45 mg/L, respectively. However, Pseudomonas oryzihabitans, Chryseobacterium indologenus and Vibrio vulnificus showed the highest accumulation of tyramine in tyrosine decarboxylase broth with values of 1648.85, 774.20 and 187.96 mg/L, respectively. Serratia liquefaciens produced more than 1000 mg/L putrescine in both enrichment broths, although Serratia rulnidace did not have the ability to produce amines (except for dopamine, serotonin and agmatine) in tyrosine decarboxylase broth. The study results show that P. mirabilis and  Enterobacter cloacae, which were dominantly found in spoiled sardine, were strong amine producers.

Keywords: Biogenic amines, histamine, tyramine, spoilage, sardine

African Journal of Biotechnology Vol. 12(21), pp. 3288-3295

Author Biographies

Abderrahmane Houicher
Department of Agriculture, Faculty of Science, Laghouat University, BP 37 G, Laghouat 03000, Algeria.
Esmeray Kuley
Department of Fishing and Fish Processing Technology, Faculty of Fisheries, Cukurova University, 01330 Balcali, Adana, Turkey
Badis Bendeddouche
High National Veterinary School, BP 161 El Harrach, Algeries 16000, Algiers
Fatih Özogul
Department of Fishing and Fish Processing Technology, Faculty of Fisheries, Cukurova University, 01330 Balcali, Adana, Turkey
Published
2016-03-14
Section
Articles

Journal Identifiers


eISSN: 1684-5315