Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using commercial products for seed treatment

  • Moacir Ribeiro Neto
  • Cíntia de Oliveira Martendal
  • Flávia Dionísio Pereira
  • Edson Luiz Souchie
  • Fabiano Guimarães Silva

Abstract

Given the current demand for timber from forest species, there is a need to develop new strategies for the mass propagation of eucalyptus. Therefore, this study aimed to determine the effect of different doses of Standak Top® and CoMo Raiz® on the establishment of Eucalyptus grandis W. Hill ex Maiden from seeds in vitro. The experimental design was completely randomized in a 4 × 3 + 1 factorial arrangement (four concentrations: 1, 2, 3, and 4 mL L-1 of the commercial products Standak Top®, CoMo Raiz®, a mixture of both, and a control treatment) with 20 replicates. The following characteristics were evaluated: Contamination level, shoot length, and the number of leaves. No contamination was observed for the different doses of Standak Top®. Additions of 1 to 3 mL L-1 of Standak Top® to the culture media yielded the greatest shoot length, and the additions of 2 to 3 mL L-1 yielded the greatest number of leaves. Mixing Standak Top® and CoMo Raiz® did not significantly enhance the measured characteristics.

Keywords: Standak Top®, CoMo Raiz®, tissue culture, asepsis, culture media

African Journal of Biotechnology Vol. 12(25), pp. 3963-3967

Author Biographies

Moacir Ribeiro Neto
Instituto Federal Goiano – Câmpus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
Cíntia de Oliveira Martendal
Universidade Estadual de Maringá, Centro de Ciências Biológicas. Av. Colombo, 5700. Câmpus Universitário, Cep. 87020-900, Maringá, Paraná, Brasil
Flávia Dionísio Pereira
Instituto Federal Goiano – Câmpus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
Edson Luiz Souchie
Instituto Federal Goiano – Câmpus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
Fabiano Guimarães Silva
Instituto Federal Goiano – Câmpus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
Published
2016-03-17
Section
Articles

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eISSN: 1684-5315