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African Journal of Biotechnology

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Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using commercial products for seed treatment

Moacir Ribeiro Neto, Cíntia de Oliveira Martendal, Flávia Dionísio Pereira, Edson Luiz Souchie, Fabiano Guimarães Silva

Abstract


Given the current demand for timber from forest species, there is a need to develop new strategies for the mass propagation of eucalyptus. Therefore, this study aimed to determine the effect of different doses of Standak Top® and CoMo Raiz® on the establishment of Eucalyptus grandis W. Hill ex Maiden from seeds in vitro. The experimental design was completely randomized in a 4 × 3 + 1 factorial arrangement (four concentrations: 1, 2, 3, and 4 mL L-1 of the commercial products Standak Top®, CoMo Raiz®, a mixture of both, and a control treatment) with 20 replicates. The following characteristics were evaluated: Contamination level, shoot length, and the number of leaves. No contamination was observed for the different doses of Standak Top®. Additions of 1 to 3 mL L-1 of Standak Top® to the culture media yielded the greatest shoot length, and the additions of 2 to 3 mL L-1 yielded the greatest number of leaves. Mixing Standak Top® and CoMo Raiz® did not significantly enhance the measured characteristics.

Keywords: Standak Top®, CoMo Raiz®, tissue culture, asepsis, culture media

African Journal of Biotechnology Vol. 12(25), pp. 3963-3967



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