RAPD-PCR molecular analysis of the threatened Cabrera’s vole populations in the Iberian Peninsula

  • Samer Alasaad
  • Konstantin V Rozhkovan
  • Dmitry M. Atopkin
  • Antonio Sánchez
  • José Garrido-García
  • Ismael Romero
  • Ramón C. Soriguer
  • Galina N. Chelomina

Abstract

Optimal management and conservation programs of the threatened Cabrera’s vole require investigating potential molecular genetic markers in the genomic background, if the few remaining fragile populations are to be conserved. A collection of 30 Cabrera’s vole representing four populations in Spain and Portugal was characterized by 134 RAPD-PCR markers. Molecular analyses did not detect low level of the genetic diversity or population bottlenecks in all studied populations, in discordance with the expectation of low level of viability of the Cabrera’s vole. The results described Cabrera’s vole populations as a single genetic unit with slightly restricted gene flow. Phylogenetic reconstruction suggested genetic differentiation between Northern and Southern Cabrera’s vole populations, with the basal branches formed by the southern populations, which may be an evidence of the southern origin of Iberian vole ancestral population. To our knowledge, this is the first study on the genetic diversity of Microtus cabrerae, which may have further application for the conservation programs of this threatened species of Iberian vole.

Keywords: Microtus cabrerae, RAPD-PCR, Spain, Portugal, gene flow, genetic diversity, bottleneck, conservation

African Journal of Biotechnology Vol. 12(26), pp. 4065-4073

Author Biographies

Samer Alasaad
Institute of Biology and Soil Science, Far Eastern Branch of the Russian, Academy of Sciences, 690022 Vladivostok, Russia; Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s/n, E-23071, Jaén, Spain; Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda.
Américo Vespucio s/n 41092 Sevilla, Spain
Konstantin V Rozhkovan
Institute of Biology and Soil Science, Far Eastern Branch of the Russian, Academy of Sciences, 690022 Vladivostok, Russia
Dmitry M. Atopkin
Institute of Biology and Soil Science, Far Eastern Branch of the Russian, Academy of Sciences, 690022 Vladivostok, Russia
Antonio Sánchez
Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s/n, E-23071, Jaén, Spain.
José Garrido-García
Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n 41092 Sevilla, Spain
Ismael Romero
Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s/n, E-23071, Jaén, Spain.
Ramón C. Soriguer
Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n 41092 Sevilla, Spain
Galina N. Chelomina
Institute of Biology and Soil Science, Far Eastern Branch of the Russian, Academy of Sciences, 690022 Vladivostok, Russia
Published
2016-03-28
Section
Articles

Journal Identifiers


eISSN: 1684-5315