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African Journal of Biotechnology

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In vitro seed germination and seedling development of the orchid Coelogyne stricta (D. Don) Schltr.

Gaurav Parmar, Bijaya Pant

Abstract


Coelogyne stricta (D. Don) Schltr., an orchid of high ornamental and medicinal values, is native to Nepal at 1400 to 2000 m elevations. In vitro seed germination and seedling development was carried out on 0.8% (w/v) agar solidified Murashige and Skoog (MS) medium, supplemented with various combinations of α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 1 mg/L BAP and 1 mg/L NAA was found to be the best condition for the development. The germination started after 7 weeks of culture and complete seedlings were obtained after 23 weeks of culture on the medium supplemented with 1 mg/L BAP and 1 mg/L NAA suggesting the usefulness of both hormones in root induction. In the hormone, free MS medium germination started after 5 weeks, but root initials were not developed even after 32 weeks of culture.

Keywords: Coelogyne stricta, in vitro, Murashige and Skoog (MS), 6 benzylaminopurine (BAP), α-naphthalene acetic acid (NAA)




http://dx.doi.org/10.5897/AJB2015.14870
AJOL African Journals Online