PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register



In vitro regenerative potentials of the medicinal plant Abutilon indicum (L.) Sweet

Sudarshana Mysore Shankarsingh, Nissar Ahmad Reshi, Girish Hodiyala Vasanaika

Abstract


The present study was undertaken to evaluate the most suitable concentration of growth regulators for callus induction and subsequent organogenesis in Abutilon indium (L.) Sweet (Malvaceae) leaf, stem and seed explants. Since the plant has a lot of medicinal importance, it was subjected to culture in vitro. Kinetin for seeds, 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) for leaf as well as BAP and kinetin for stem were the growth regulators used at different concentrations individually. Results showed that growth regulators at different combinations on subculture resulted in the regeneration of shoots. The excised shoots were transferred to liquid medium for in vitro rhizogenesis and plantlets were acclimatized in the laboratory conditions before they were transferred to garden soil. Histologically, callus sections were with active centres, embryoids and multiple shoot buds. Cytological variations such as multinucleate, chromosome bridges, polyploidy and tracheary elements were noticed in callus cells. The regenerated plants were morphologically and cytologically similar to the in vivo plants.

Key words: Embryoids, multiple shoot buds, multinucleate, polyploidy, Malvaceae.




http://dx.doi.org/10.5897/AJB2015.14887
AJOL African Journals Online