Evidence of cross gene regulation of some virulence factors of Porphyromonas gingivalis by Streptococcus intermedius

  • Victor E Aguirre-Arzola
  • Benito Pereyra-Alferez
  • Rosa Isela Sanchez-Najera
  • Luis J Galan-Wong
  • Myriam A. De La Garza-Ramos
  • Andrea G. Alcazar-Pizaña

Abstract

Periodontal disease has been associated with poor dental care, which promotes the accumulation of bacteria and the development of diseases of the mouth. Porphyromonas gingivalis are anaerobic Gramnegative bacteria found in the subgingival plaque. They are largely responsible for chronic periodontal disease. Streptococcus intermedius is a Gram positive coccus found in the supragingival plaque. The objective of the present work was to evaluate the expression of several virulence genes of P. gingivalis in a mixed culture with S. intermedius using qPCR and heterologous microarrays. P. gingivalis ATCC 33277 and W83 and S. intermedius ATCC 27335 strains were cultured and total RNA was extracted using the High Pure RNA isolation kit. Oligodeoxynucleotides were designed to make multiple comparisons with organisms. Microarray was performed to identify gene expression. To quantify gene expression, cDNA samples from three different P. gingivalis:S. intermedius ratios were diluted 10-1, 10-2 and 10-3. The microarray experiment indicated that in P. gingivalis, 29 genes were upregulated. The putative function of upregulated genes was the biosynthesis of different metabolic pathways. Heterologous microarrays are a new approach that are useful for investigating gene expression.

Keywords: Porphyromonas gingivalis, Streptococcus intermedius, periodontal disease, virulence genes, cross gene regulation.

African Journal of Biotechnology Vol. 12(28), pp. 4498-4502

Author Biographies

Victor E Aguirre-Arzola
Instituto de Biotecnología/Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo Leon, Monterrey, Mexico.
Benito Pereyra-Alferez
Instituto de Biotecnología/Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo Leon, Monterrey, Mexico.
Rosa Isela Sanchez-Najera
Facultad de Odontología/Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo Leon, Monterrey, Mexico
Luis J Galan-Wong
Instituto de Biotecnología/Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo Leon, Monterrey, Mexico.
Myriam A. De La Garza-Ramos
Facultad de Odontología/Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo Leon, Monterrey, Mexico
Andrea G. Alcazar-Pizaña
Facultad de Odontología/Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo Leon, Monterrey, Mexico
Published
2016-04-06
Section
Articles

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eISSN: 1684-5315