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African Journal of Biotechnology

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Molecular variance of the Tunisian almond germplasm assessed by simple sequence repeat (SSR) markers

Hassouna Gouta, Elhem Ksia, Ahmed Mliki, Yolanda Gogorcena

Abstract


The genetic variance analysis of 82 almond (Prunus dulcis Mill.) genotypes was performed using ten genomic simple sequence repeats (SSRs). A total of 50 genotypes from Tunisia including local landraces identified while prospecting the different sites of Bizerte and Sidi Bouzid (Northern and central parts) which are the most important locations of almond diversity in Tunisia were included. Analysis of molecular variance (AMOVA) was  performed for 11 populations from these different regions and foreign countries to examine the distribution of genetic variation of the accessions studied. Results show that the major variation occurred within populations in each geographic site. Additionally, this analysis demonstrates that the genetic diversity within local almond cultivars was important, with a clear geographic distinction between the Northern and the Southern Tunisian cultivars. The value of prospecting new sites, preserving genetic diversity and encouraging on farmers almond collections is also discussed.

Keywords: Prunus dulcis Mill., Genetic resources, AMOVA, local ecotypes, geographic origin, Tunisia

African Journal of Biotechnology Vol. 12(29), pp. 4569-4577



http://dx.doi.org/10.5897/AJB2013.12501
AJOL African Journals Online