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African Journal of Biotechnology

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Comparison of in vitro morphogenetic capacities of different clones of three local cultivars of sweet potato (Ipomoea batatas) from Togo

Glato Kodjo, Etse Kodjo Djidjolé, Sanvee Mensah, Tozo Koffi, Aidam Atsou

Abstract


Sweet potato (Ipomoea batatas) is an hexaploide plant with complex incompatibility that reduces chances of self-fertilization. In vitro culture of sweet potato by means of biotechnological approaches is an advantage for its study and variety improvement. Three sweet potato cultivars from Togo: Damadoami, Tombolo, Nagohé were grown on Murashige and Skoog (MS) medium with or without indole-3-butyric acid (IBA), and the morphogenetic properties of the plantlets obtained were compared. Uninodal stem explants were used. The reactivity of explants grown on MS differed according to the cultivar and the clone. Addition of IBA induced embryogenesis and root neoformations. The growth depended on the genotype, the clone, and the cultivar. The cultivar Damadoami was the most reactive on MS medium. Plantlets were acclimated easily and can be transferred to the field

Keywords: Tuberous root, in vitro, morphogenesis, neoformations, indole-3-butyric acid (IBA), Togo.

African Journal of Biotechnology Vol. 12(29), pp. 4648-4655



http://dx.doi.org/10.5897/AJB2013.12083
AJOL African Journals Online