Alkaline pretreatment of Mexican pine residues for bioethanol production

  • Claudia Elena Soto Alvarez
  • Javier López Miranda
  • Gustavo Pérez Verdín
  • Mario A. Rodríguez Pérez
  • Isaías Chairez Hernández


The locally sourced residue samples of Pinus arizonica, Pinus cooperi, and Pinus durangensis from the state of Durango in Mexico were analyzed for optimal yield of ethanol production. The samples were mixed at an equal proportion using a particle size of 0.59 mm. Each individual mixture was pretreated with either NaOH or Ca (OH)2 (at 0.5, 1.0 and 1.5% w/v) for periods of 30, 60, and 90 min at 60, 90, and 120°C. The pretreated blending was subjected to enzymatic hydrolysis for 130 h at 80 rpm and 50°C with an enzymatic load of 25 filter paper units (FPU) and 50 IU β-glucosidase per gramme of cellulose to obtain a maximum yield of reducing sugars (RS) with NaOH subject at 120°C for 90 min. The results show that the hydrolysis yield depends on temperature and alkali concentration particularly (NaOH), which increased from 2.0 to 3.5% w/v. The best yield of glucose (41.33% w/w) was obtained using a pretreatment of 2.5% NaOH for 90 min, 120°C, and a hydrolysis residence time of 130 h. The removal of lignin and hemicellulose acetylation was observed to have influence on the enzymatic digestibility of cellulose. This process could theoretically produce a maximum yield of 90.19% of ethanol / substrate (glucose) and about 80 L of bioethanol per dry ton of woody biomass from pine residues.

Keywords: Lignocellulosic biomass, alkaline pretreatment, enzymatic hydrolysis, fermentable sugars, fermentation

African Journal of Biotechnology Vol. 12(31), pp. 4956-4965

Author Biographies

Claudia Elena Soto Alvarez
Instituto Politécnico Nacional, CIIDIR-DGO. 34220, Durango, Dgo., México
Javier López Miranda
Instituto Tecnológico de Durango. 34080, Durango, Dgo., México.
Gustavo Pérez Verdín
Instituto Politécnico Nacional, CIIDIR-DGO. 34220, Durango, Dgo., México.
Mario A. Rodríguez Pérez
Instituto Politécnico Nacional, Centro de Biotecnología Genómica. 88710, Reynosa, Tamaulipas, México
Isaías Chairez Hernández
Instituto Politécnico Nacional, CIIDIR-DGO. 34220, Durango, Dgo., México.

Journal Identifiers

eISSN: 1684-5315