Molecular and functional diversity in Capsicum landraces of Andaman Islands

  • Shrawan Singh
  • DR Singh
  • R Balaji
  • Dipak Nayak
  • AK Singh
  • Naresh Kumar

Abstract

The present study analyzed the diversity in 26 landraces of Capsicum from Andaman Islands using 20 morphological, 16 biochemical and 10 DNA markers. Significant differences were observed in tested landraces and 16 reference genotypes from mainland India. Biochemical markers grouped all the genotypes into eight clusters with inter-cluster distance of 0.5 to 1.9 while seven quantified morphological traits divided the test genotypes into three major clusters and seven sub-clusters with 0.1 to 1.6 inter-cluster distance value. The random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers assured the genetic nature of diversity in landraces. The similarity matrix from RAPD and ISSR markers revealed 48% diversity among 42 genotypes with polymorphism information content (PIC) values of 0.43 and 0.41, respectively. The correspondence in morphological and biochemical markers indicates their interdependence for observed traits. However, poor correlation between DNA profiles and functional markers suggest further screening of more number of markers.The study identified phytochemical rich landraces CA-334, SPG-7, CARI-1 and CCB-2. The information will be useful in chemo-taxonomic foot-printing of Capsicum landraces and devising apposite conservation and utilization strategies.

Keywords: Capsicum, landraces, functional diversity, chemo-taxonomic diversity, DNA markers

African Journal of Biotechnology Vol. 12(39), pp. 5729-5737

Author Biographies

Shrawan Singh
Division of Horticulture and Forestry, Central Agricultural Research Institute, Port Blair-744101, Andaman and Nicobar Islands, India
DR Singh
Division of Horticulture and Forestry, Central Agricultural Research Institute, Port Blair-744101, Andaman and Nicobar Islands, India
R Balaji
Division of Horticulture and Forestry, Central Agricultural Research Institute, Port Blair-744101, Andaman and Nicobar Islands, India
Dipak Nayak
Division of Horticulture and Forestry, Central Agricultural Research Institute, Port Blair-744101, Andaman and Nicobar Islands, India
AK Singh
Division of Horticulture and Forestry, Central Agricultural Research Institute, Port Blair-744101, Andaman and Nicobar Islands, India
Naresh Kumar
Division of Horticulture and Forestry, Central Agricultural Research Institute, Port Blair-744101, Andaman and Nicobar Islands, India
Published
2016-05-16
Section
Articles

Journal Identifiers


eISSN: 1684-5315