Decolourization of remazol brilliant blue R by enzymatic extract and submerged cultures of a newly isolated Pleurotus ostreatus MR3

  • Fengxue Xin
  • Yumeng Sun
  • Siyao Hu
  • Karlai Cheong
  • Anli Geng

Abstract

A local white-rot fungus basidiomycete Pleurotus ostreatus MR3 was isolated from MacRitchie Reservoir Park, Singapore. Among all the ligninolytic activities, laccase was the only enzyme detected in the supernatant when the fungus was grown in liquid culture. This newly isolated white rot fungus was able to completely decolourise remazol brilliant blue R (RBBR) in-vivo on agar plates within five days and in the liquid culture (in the presence of inducers) within three days. The addition of inducers was able to enhance laccase production and therefore enhanced in-vivo RBBR decolourisation. Veratryl alcohol was shown to be the best inducer for laccase production with the maximum laccase activity reaching about 5.99 U/mL. Cu2+ also had a positive effect on laccase production, the laccase activity being enhanced to 5.24 U/mL. In-vitro RBBR decolourisation using the laccase from P. ostreatus MR3 was much comparable to that using the commercial laccase from Trameters versicolor.

Keywords: Dyes, remazol brilliant blue R, Pleurotus ostreatus MR3, decolourisation, inducers, laccase activity

African Journal of Biotechnology Vol. 12(39), pp. 5778-5783

Author Biographies

Fengxue Xin
School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore 599489
Yumeng Sun
Hwa Chong Junior College, Singapore.
Siyao Hu
Hwa Chong Junior College, Singapore.
Karlai Cheong
Hwa Chong Junior College, Singapore.
Anli Geng
School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore 599489.
Published
2016-05-16
Section
Articles

Journal Identifiers


eISSN: 1684-5315