Bacterial species identification getting easier

  • Phumudzo Tshikhudo
  • Ronald Nnzeru
  • Khayalethu Ntushelo
  • Fhatuwani Mudau

Abstract

The traditional methods of bacterial identification are based on observation of either the morphology of single cells or colony characteristics. However, the adoption of newer and automated methods offers advantage in terms of rapid and reliable identification of bacterial species. The review provides a comprehensive appreciation of new and improved technologies such fatty acid profiling, sequence analysis of the 16S rRNA gene, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, together with the computational tools employed for querying the databases that are associated with these identification tools and high-throughput genomic sequencing in bacterial identification. It is evident that with the increase in the adoption of new technologies bacterial identification is becoming easier.

Keywords: Bacteria, Biolog, computational tools, fatty acids, Gram staining, identification, metagenomics, morphology, MALDI-TOF MS, RiboPrinter, 16S rRNA gene.

African Journal of Biotechnology Vol. 12(41), pp. 5975-5982

Author Biographies

Phumudzo Tshikhudo
University of South Africa, Department of Agriculture and Animal Health, Private Bag X6, Florida, 1710, South Africa
Ronald Nnzeru
University of South Africa, Department of Agriculture and Animal Health, Private Bag X6, Florida, 1710, South Africa
Khayalethu Ntushelo
University of South Africa, Department of Agriculture and Animal Health, Private Bag X6, Florida, 1710, South Africa
Fhatuwani Mudau
University of South Africa, Department of Agriculture and Animal Health, Private Bag X6, Florida, 1710, South Africa
Published
2016-06-06
Section
Articles

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eISSN: 1684-5315