Random amplified polymorphic DNA (RAPD) markers reveal genetic diversity in bael (Aegle marmelos Correa) genotypes of Andaman Islands, India

  • Dipak Nayak
  • DR Singh
  • P Sabarinathan
  • Shrawan Singh
  • Tarama Nayak

Abstract

The present study evaluated genetic variability of superior bael genotypes collected from different parts of Andaman Islands, India using fruit characters and random amplified polymorphic DNA (RAPD) markers. Genomic DNA extracted from leaf material using cetyl trimethyl ammonium bromide (CTAB) method was subjected to polymerase chain reaction (PCR) with 12 polymorphic primers. A total of 476 polymorphic loci were identified with mean value of 39.66 bands per primer and 63.99% polymorphism. Application of unweighted pair group method using arithmetic average cluster analysis generated three genotypic groups. ‘Bael-5’ and ‘Bael-8’ were most similar genotypes whereas ‘Bael-7’ and ‘Bael-1’ were extreme divergent. The clusters based on molecular data were not in agreement with the morphological traits in most of the cases as low level of correlation was observed between the classification methods based on fruit characteristics and RAPD markers. The bael genotypes were found to have considerable genetic variability, demonstrating the importance of RAPD markers to analyse each genotype in a collection in order to efficiently maintain the germplasm collection for genetic improvement of bael.

Keywords: Aegle, Bael, random amplified polymorphic DNA (RAPD) and genetic diversity.

African Journal of Biotechnology Vol. 12(42), pp. 6055-6060

Author Biographies

Dipak Nayak
Indian Agricultural Research Institute- Regional Station, Kalimpong, West Bengal, India- 734 301.
DR Singh
Central Agricultural Research Institute, Port Blair, A and N Islands, India- 744 101.
P Sabarinathan
Bharat College of Science and Management, Thanjavur, Tamil Nadu, India- 613 005.
Shrawan Singh
Central Agricultural Research Institute, Port Blair, A and N Islands, India- 744 101.
Tarama Nayak
Department of ASEPAN, Palli Siksha Bhavan, Visva Bharati, Santiniketan, West Bengal, 731 235- India.
Published
2016-06-06
Section
Articles

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eISSN: 1684-5315