Growth pattern and structural nature of amylases produced by some Bacillus species in starchy substrates
The growth pattern and microbial biomass formed during metabolic activities of the Bacillus species on starchy substrates was determined. The result showed that the strains B. subtilis (WBS), B. licheniformis (WBL) and B. coagulans (MBC) generally had high growth rate. B. circulans (SBC) and B. coagulans (WBC) has specific affinity for growth and some enzymatic activity on corn starch medium compared with other lower growth observed in B. polymyxa (WBP) but higher enzyme production. The amylolytic Bacillus species obtained utilized white corn starch substrate as a sole carbon source as well as soluble starch. The amylase production values range from 0.022 x 102 unit/cfu in B. circulans (WBC) to 0.912 x 102 unit/cfu in B. licheniformis (WBL) for corn starch, and 0.01 x 102 unit/cfu in both B. megaterium (SBG) and B. licheniformis (SBL) to 0.693 x 102 unit/cfu in B. subtilis (WBS) for soluble starch.
Keywords: Activity, Bacillus, enzymatic, metabolic, starchy, substrate