Aphid resistance in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) induced by sea anemone equistatin overexpression

  • Mahmood Valizadeh
  • Celine Deraison
  • Seyed Kamal Kazemitabar
  • Yvan Rahbé
  • Maarten A Jongsma

Abstract

Florist's chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Most genotypes are sensitive to aphids and infestations can lower quality and cause transmission of viruses. The protease inhibitor Sea Anemone Equistatin (SAE) carries three domains responsible for the inhibition of both cysteine and aspartic proteases. Artificial diet bioassays showed that SAE is readily toxic when ingested by the pea aphid, Acyrthosiphon pisum, and the cotton aphid, Aphis gossypii. We transformed chrysanthemum genotype 1581 by Agrobacterium tumefaciens-mediated transformation with the SAE gene under the control of the chrysanthemum RbcS promoter to induce aphid resistance. Non-choice leaf disk and whole plant bioassays were carried out to analyze deleterious effects of SAE on population growth and survival of both Myzus persicae and A. gossypii. After 7 days, M. persicae populations on specific transgenic lines were up to 69% smaller relative to control populations in a whole plant bioassay. The mortality of cotton aphids was 11% on control lines and up to 32% on transgenic lines after 5 days. The results show that SAE may be a promising agent for the control of some aphid species in transgenic plants.

Keywords: Chrysanthemum morifolium, aphid resistance, RbcS promoter, sea anemone equistatin, agrobacterium transformation.

Afr. J. Biotechnol. Vol. 12 No. 50

Author Biographies

Mahmood Valizadeh

Department of Agriculture, Payame Noor University, Kashmar Branch, Kashmar, Iran; Plant Research International, Wageningen University and Research Center, P. O. Box 619, 6700 AP Wageningen, Netherlands

Celine Deraison
INRA-INSA de Lyon, UMR BF2I (Biologie Fonctionelle Insectes & Interactions), 20 ave A. Einstein 69621, Villeurbanne Cedex, France
Seyed Kamal Kazemitabar
Department of Plant Breeding, Sari Natural Resources and Agricultural University, Sari, Mazandaran, Iran
Yvan Rahbé
INRA-INSA de Lyon, UMR BF2I (Biologie Fonctionelle Insectes & Interactions), 20 ave A. Einstein 69621, Villeurbanne Cedex, France
Maarten A Jongsma
Plant Research International, Wageningen University and Research Center, P. O. Box 619, 6700 AP Wageningen, Netherlands
Published
2016-07-17
Section
Articles

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eISSN: 1684-5315