Production of L-glutaminase and its optimization from a novel marine isolate Vibrio azureus JK-79

  • Jambulingam Kiruthika
  • Nachimuthu Saraswathy

Abstract

L-Glutaminase is an amidohydrolase which is produced by a variety of micro-organisms including bacteria, yeast and fungi. It is currently used in the treatment of acute lymphoblastic leukemia (ALL) and human immunodeficiency virus (HIV). Another potential application of L-glutaminase is as flavor enhancing agent in soy sauce fermentation. Even though L-glutaminase activity was reported in numerous micro-organisms, production of L-glutaminase from marine bacteria is very scanty. With this view, in the present research work, L-glutaminase production pattern was studied under submerged fermentation using novel marine isolate Vibrio azureus strain JK-79 (GenBank Acession Number JQ820323) based on one-factor-at-a-time approach. The maximum yield of enzyme production (247 U/ml) was achieved in a seawater based medium at pH 8, 37°C, 1% inoculum concentration and 2% glutamine concentration for 24 h. The medium when supplemented with carbon source, it improved the enzyme production from 247 to 321 U/ml with 1.5% maltose. Addition of 2% soybean meal also improved the L-glutaminase production (289 U/ml). The above results indicate the scope for production of salt tolerant L-glutaminase using this novel marine bacterial strain.

Keywords: L-Glutaminase, Vibrio azureus JK-79, submerged fermentation, optimization of fermentation, one-factor-at-a-time approach.

Afr. J. Biotechnol Vol. 12 No. 50

Author Biographies

Jambulingam Kiruthika
Government College of Technology, Coimbatore, Tamil Nadu, INDIA
Nachimuthu Saraswathy
Kumaraguru college of Technology,Coimbatore, Tamil Nadu, INDIA
Published
2016-07-17
Section
Articles

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eISSN: 1684-5315