Emerging Acinetobacter schindleri in red eye infection of Pangasius sutchi

  • M. Radha Krishna Reddy
  • SA Mastan

Abstract

This communication provides an insight into the emerging of new infection “red eye” in Pangasius sutchi and aimed to screen the prime pathogens involved in disease. The pathogen was isolated from diseased P. sutchi and characterized by morphological, biochemical and molecular approach, which includes 16s r RNA gene sequencing. Polymerase chain reaction (PCR) amplified 16s RNA was separated using agarose gel electrophoresis, eluted product was sequenced and BLAST analysis was carried out to identify the pathogens. Identified virulent bacterial strain Acinetobacter schindleri with LD50 108.35 initiated re-infection in experimentally in infected Pangasius fingerlings. This study provided the evidence of A. schindleri which is true causative agents in red eye disease in P. sutchi. To the best of knowledge of this study, there was no track record of A. schindleri eye infection in fishes till date around the globe.

Keywords: Pangasius sutchi, 16s r- RNA gene sequencing, Acinetobacter schindleri, LD50.

Afr. J. Biotechnol. Vol. 12 No. 50

Author Biographies

M. Radha Krishna Reddy
Department of Biotechnology, Krishna University, Machilipatnam-521001, Andhra Pradesh, India
SA Mastan
P.G Department  of Biotechnology, PG Courses, Research Center, DNR College, Bhimavaram- 534202, Andhra Pradesh, India
Published
2016-07-17
Section
Articles

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eISSN: 1684-5315