In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (l.) Del.

  • Mansor Ndoye Laboratoire de biotechnologies, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop Dakar, B.P. 5005, Dakar, Sénégal
  • Ismaïla Diallo ISRA-DRPF, Route des Pères Maristes, B.P. 2312, Dakar, Sénégal
  • Yaye Kène Gassama/Dia Laboratoire de biotechnologies, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop Dakar, B.P. 5005, Dakar, Sénégal

Abstract

Procedures were developed for micropropagation of Balanites aegyptiaca using axillary bud explants obtained from mature trees. Cultures were established in Murashige and Skoog (MS) medium supplemented with 2.5 mg/l 6-benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA). The effects of kinetin on shoot growth and proliferation in vitro was also investigated. Results show that shoot multiplication required 2.5 mg/l of BAP. Shoot length was significantly affected by the presence of BAP or 6-furfurylaminopurine (Kin). Rooting of shoots in vitro was achieved on MS medium containing 20 mg/l of the auxin, indolebutyric acid (IBA). Rooted shoots acclimated and were successfully transferred into soil, with 48% of the plantlets surviving.

Key words: Balanites aegyptiaca, micropropagation, rooting.

African Journal of Biotechnology Vol.2(11) 2003: 421-424

Author Biography

Mansor Ndoye, Laboratoire de biotechnologies, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop Dakar, B.P. 5005, Dakar, Sénégal
Tel: 5331084. Fax: (221) 824 63 18.
Published
2004-02-27
Section
Articles

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eISSN: 1684-5315